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<div id="lvtttdx" class="appMask"></div> <div id="lvtttdx" class="appContent"> <div id="lvtttdx" class="clearfix product_info_pose_4" id="phx_product_info" data-product-id="2022339893" data-host="m.e4522.cn" data-dffixationtitle="EPP化學(xué)發(fā)光試劑盒用途"> <div id="lvtttdx" class="info"> <div id="lvtttdx" class="magnifying"> <div id="lvtttdx" class="phx-imagezoom clearfix"> <div id="lvtttdx" class="tb-booth tb-pic tb-s310"> <div id="lvtttdx" class="img-wrapper" data-type="productbig"> <a href="javascript:void(0);" data-src="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast" data-rel="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast" data-title=""></a> </div> <a class="big_photo" href="/View_Image_2022339893.html" target="_blank">查看大圖</a> </div> <ul class="info_list"> <li><h1 class="info_name">產(chǎn)品名稱：人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒</h1></li> <li>產(chǎn)品型號(hào)：EPP</li> <li>產(chǎn)品廠商：KALANG</li> <li>產(chǎn)品文檔： </li> </ul> <ul class="tb-thumb thumblist"> <li id="lvtttdx" class="tb-selected"> <div id="lvtttdx" class="tb-pic tb-s40 img-wrapper" data-type="productsmall" data-mid="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast" data-big="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast"> <a data-src="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast"></a> </div> </li> </ul> <div id="lvtttdx" class="buy-tips"> 你添加了1件商品 <a href="http://m.e4522.cn/member/order_cart.aspx">查看購(gòu)物車</a> </div> </div> </div> </div> <div id="lvtttdx" class="simple_intro"> <div id="lvtttdx" class="title"> 簡(jiǎn)單介紹： </div> <div id="lvtttdx" class="text"> 人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒采用競(jìng)爭(zhēng)CLIA法。用人EPP抗原包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的EPP與包被的EPP競(jìng)爭(zhēng)生物素標(biāo)記的抗人EPP單抗上的結(jié)合位點(diǎn)，游離的成分被洗去。加入辣根過氧化物酶標(biāo)記的親和素，生物素與親和素特異性結(jié)合而形成**復(fù)合物，游離的成分被洗去。加入發(fā)光底物混合液，發(fā)光底物在辣根過氧化物酶的催化下發(fā)出熒光，用化學(xué)發(fā)光**分析儀測(cè)定化學(xué)發(fā)光值（RLU），人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒濃度與化學(xué)發(fā)光值之間呈負(fù)相關(guān)，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中EPP的濃度。 </div> </div> <div id="lvtttdx" class="details_info"> <div id="lvtttdx" class="title"> 詳情介紹： </div> <div id="lvtttdx" class="text yzeditor"> <p> <strong>人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒</strong> </p> <p> 基本原理： </p> <p> <span style="font-family:Verdana, Arial, Helvetica, sans-serif;line-height:20px;text-align:justify;text-indent:24px;white-space:normal;">本試劑盒<span style="font-family:Verdana, Arial, Helvetica, sans-serif;font-size:13.3333px;line-height:20px;text-align:justify;text-indent:26.6667px;white-space:normal;">采用競(jìng)爭(zhēng)CLIA法。用人EPP抗原包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的EPP與包被的EPP競(jìng)爭(zhēng)生物素標(biāo)記的抗人EPP單抗上的結(jié)合位點(diǎn)，游離的成分被洗去。加入辣根過氧化物酶標(biāo)記的親和素，生物素與親和素特異性結(jié)合而形成**復(fù)合物，游離的成分被洗去。加入發(fā)光底物混合液，發(fā)光底物在辣根過氧化物酶的催化下發(fā)出熒光，用化學(xué)發(fā)光**分析儀測(cè)定化學(xué)發(fā)光值（RLU），EPP濃度與化學(xué)發(fā)光值之間呈負(fù)相關(guān)，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中EPP的濃度。</span></span> </p> <p> <span style="white-space:normal;"><strong style="white-space:normal;"><strong style="white-space:normal;"><strong>人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒</strong></strong></strong>描述：</span> </p> <p> <table class=" noBorderTable ke-zeroborder" align="center" border="0" cellspacing="1" width="640" style="margin:0px;padding:0px;color:#5D5D5D;font-family:Verdana, Arial, Helvetica, sans-serif;font-size:13.3333px;background-color:#A8C29F;"> <tbody style="margin:0px;padding:0px;"> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">英文名稱</span> </td> <td colspan="3" bgcolor="#ffffff" height="25" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">Human EPP (Erythrocyte Protoporphyrin) CLIA Kit</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">中文名稱</span> </td> <td height="25" colspan="3" bgcolor="#ffffff" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">貨號(hào)</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">KL-H1417c</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">種屬</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">Human</span>/人 </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">規(guī)格</span> </td> <td height="25" colspan="3" bgcolor="#ffffff" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">96T/Kit (8*12 wells)</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">檢測(cè)方法</span> </td> <td height="25" colspan="3" bgcolor="#ffffff" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">競(jìng)爭(zhēng)法</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">檢測(cè)范圍</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">0.625~40ng/mL</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">靈敏度</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="lvtttdx" class="black" style="margin:0px;padding:0px;color:#000000;">0.375ng/mL</span> </td> </tr> </tbody> </table> </p> <p> <strong style="line-height:1.5;"><strong style="white-space:normal;">人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒</strong></strong><span style="line-height:1.5;">試劑盒組成：</span> </p> <p><table class=" <tbody> <tr> <td bgcolor="#ffffff" width="160"> <strong><span id="lvtttdx" </td> <td bgcolor="#ffffff" <strong><span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <strong><span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <strong><span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <br> </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="400"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="200"> <span id="lvtttdx" </td> <td bgcolor="#ffffff" width="160"> <br> </td> </tr> <tr> <td bgcolor="#ffffff"> <span id="lvtttdx" </td> <td bgcolor="#ffffff"> <span id="lvtttdx" </td> <td bgcolor="#ffffff"> <span id="lvtttdx" </td> <td bgcolor="#ffffff"> <br> </td> </tr> <tr> <td colspan="4" bgcolor="#ffffff"> <strong><span id="lvtttdx" <span id="lvtttdx" </td> </tr> </tbody> </table> <strong style="white-sp noBorderTable ke-zeroborder" align="center" border="0" cellspacing="1" width="650" style="margin:0px;padding:0px;color:#5D5D5D;font-family:Verdana, Arial, Helvetica, sans-serif;background-color:#A8C29F;"> class="black" style="color:#000000;">中文名稱</span></strong> height="25" width="400"> class="black" style="color:#000000;">英文名稱</span></strong> class="black" style="color:#000000;">規(guī)格</span></strong> class="black" style="color:#000000;">保存</span></strong> class="black" style="color:#000000;">CLIA酶標(biāo)板</span> class="black" style="color:#000000;">Micro CLIA Plate</span> class="black" style="color:#000000;">8×12 wells</span> class="black" style="color:#000000;">4℃/-20℃ #</span> class="black" style="color:#000000;">凍干標(biāo)準(zhǔn)品</span> class="black" style="color:#000000;">Reference Standard</span> class="black" style="color:#000000;">2 支</span> class="black" style="color:#000000;">4℃/-20℃ #</span> class="black" style="color:#000000;">標(biāo)準(zhǔn)品&樣品稀釋液</span> class="black" style="color:#000000;">Reference Standard & Sample Diluent</span> class="black" style="color:#000000;">1瓶 20mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">濃縮生物素化抗體</span> class="black" style="color:#000000;">Concentrated Biotinylated Detection Ab</span> class="black" style="color:#000000;">1支 120μL</span> class="black" style="color:#000000;">4℃/-20℃ #</span> class="black" style="color:#000000;">生物素化抗體稀釋液</span> class="black" style="color:#000000;">Biotinytated Detection Ab Diluent</span> class="black" style="color:#000000;">1瓶10mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">濃縮HRP酶結(jié)合物</span> class="black" style="color:#000000;">Concentrated HRP Conjugate</span> class="black" style="color:#000000;">1支 120μL</span> class="black" style="color:#000000;">4℃(避光)</span> class="black" style="color:#000000;">酶結(jié)合物稀釋液</span> class="black" style="color:#000000;">HRP Conjugate Diluent</span> class="black" style="color:#000000;">1瓶 10mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">濃縮洗滌液（25×）</span> class="black" style="color:#000000;">Concentrated Wash Buffer (25×)</span> class="black" style="color:#000000;">1瓶 30mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">發(fā)光底物A液</span> class="black" style="color:#000000;">Substrate Reagent A</span> class="black" style="color:#000000;">1瓶 5mL</span> class="black" style="color:#000000;">4℃(避光)</span> class="black" style="color:#000000;">發(fā)光底物B液</span> class="black" style="color:#000000;">Substrate Reagent B</span> class="black" style="color:#000000;">1瓶 5mL</span> class="black" style="color:#000000;">4℃(避光)</span> class="black" style="color:#000000;">封板覆膜</span> class="black" style="color:#000000;">Plate Sealer</span> class="black" style="color:#000000;">5 張</span> class="black" style="color:#000000;">產(chǎn)品說明書</span> class="black" style="color:#000000;">Manual</span> class="black" style="color:#000000;">1 份</span> class="black" style="color:#000000;">質(zhì)檢報(bào)告</span> class="black" style="color:#000000;">Certificate of Analysis</span> class="black" style="color:#000000;">1 份</span> class="black" style="color:#000000;">特別說明</span></strong>：<br> class="black" style="color:#000000;">#: 一周內(nèi)使用可存于4℃，需長(zhǎng)時(shí)間存放或多次使用建議存于-20℃.</span> ace:normal;"><strong style="white-space:normal;">人原卟啉(EPP)化學(xué)發(fā)光**分析試劑盒</strong></strong>操作步驟：</p> <p> 1.在各孔中加入標(biāo)準(zhǔn)品或樣品各 100μL，37℃孵育90分鐘<br> 2. 倒去孔內(nèi)液體，拍干，加入 100μL 生物素化抗體工作液，37℃孵育 60 分鐘<br> 3. 洗滌 3 次<br> 4. 加入 100μL 酶結(jié)合物工作液， 37℃孵育 30 分鐘<br> 5. 洗滌 5 次<br> 6. 加入 100μL 發(fā)光底物混合液， 37℃孵育 5 分鐘左右<br> 7. 立即測(cè)定各孔的化學(xué)發(fā)光值<br> 8. 結(jié)果計(jì)算 </p> <p> KL-ACP3-Hu 人前列腺酸性磷酸酶(ACP3) 檢測(cè)試劑盒 human Acid Phosphatase 3, Prostatic (ACP3) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.054ng/mL 96T 12 months<br> KL-TGFb1-c 犬轉(zhuǎn)化生長(zhǎng)因子β1(TGFβ1) 檢測(cè)試劑盒 Canine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit Canis familiaris; Canine Dog 15.625-1000pg/mL 5.6pg/mL 96T 12 months<br> KL-FABP8-Hu 人髓鞘型脂肪酸結(jié)合蛋白(FABP8) 檢測(cè)試劑盒 human Fatty Acid Binding Protein 8, Myelin (FABP8) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.062ng/mL 96T 12 months<br> KL-COL3-Hu 人Ⅲ型膠原(COL3) 檢測(cè)試劑盒 human Collagen Type III (COL3) ELISA Kit Homo sapiens human 0.625-40ng/mL 0.255ng/mL 96T 12 months<br> KL-COL4-Hu 人Ⅳ型膠原(COL4) 檢測(cè)試劑盒 human Collagen Type IV (COL4) ELISA Kit Homo sapiens human 0.781-50ng/mL 0.33ng/mL 96T 12 months<br> KL-ELA2-Mu 小鼠中性粒細(xì)胞彈性蛋白酶(ELA2) 檢測(cè)試劑盒 mouse Elastase 2, Neutrophil (ELA2) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.063ng/mL 96T 12 months<br> KL-HA-Ge 透明質(zhì)酸(HA) 檢測(cè)試劑盒 general Hyaluronic Acid (HA) ELISA Kit General 7.41-600 ng/mL 2.5 ng/mL 96T 12 months<br> KL-GLb-Mu 小鼠半乳糖苷酶β(GLβ) 檢測(cè)試劑盒 mouse Galactosidase Beta (GLb) ELISA Kit Mus musculus mouse 1.563-100 U/L 1.06 U/L 96T 12 months<br> KL-PK-Mu 小鼠丙酮酸激酶(PK) 檢測(cè)試劑盒 mouse Pyruvate Kinase, Liver And RBC (PK) ELISA Kit Mus musculus mouse 1.563-100 U/L 1 U/L 96T 12 months<br> KL-TREM1-Hu 人髓細(xì)胞觸發(fā)受體1(TREM1) 檢測(cè)試劑盒 human Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) ELISA Kit Homo sapiens human 15.625-1000pg/mL 5.6pg/mL 96T 12 months<br> KL-CAV1-Ra 大鼠窖蛋白(CAV1) 檢測(cè)試劑盒 Rat Caveolin 1 (CAV1) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.055ng/mL 96T 12 months<br> KL-COL1a2-Rb 兔Ⅰ型膠原α2(COL1α2) 檢測(cè)試劑盒 Rabbit Collagen Type I Alpha 2 (COL1a2) ELISA Kit Oryctolagus cuniculus Rabbit 0.313-20ng/mL 0.104ng/mL 96T 12 months<br> KL-a1M-Si 猴α1-微球蛋白(α1M) 檢測(cè)試劑盒 Monkey Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit Rhesus monkey Simian 7.813-500 ng/mL 4.5 ng/mL 96T 12 months<br> KL-TGFb2-c 犬轉(zhuǎn)化生長(zhǎng)因子β2(TGFβ2) 檢測(cè)試劑盒 Canine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit Canis familiaris; Canine Dog 15.625-1000pg/mL 5.4pg/mL 96T 12 months<br> KL-IFNb-Ra 大鼠干擾素β(IFNβ) 檢測(cè)試劑盒 Rat Interferon Beta (IFNb) ELISA Kit Rattus norvegicus Rat 15.625-1000pg/mL 6.1pg/mL 96T 12 months<br> KL-ANP-Mu 小鼠心鈉肽(ANP) 檢測(cè)試劑盒 mouse Atrial Natriuretic Peptide (ANP) ELISA Kit Mus musculus mouse 7.813-500pg/mL 3.1pg/mL 96T 12 months<br> </p> </div> </div> </div> </div> </div> <div id="lvtttdx" class="appWrapper" id="app_33341434" role="draggable" data-type="app" data-appid="message" data-config="" data-state="true"> <div id="lvtttdx" class="appMask"></div> <div id="lvtttdx" class="appContent"> <div id="lvtttdx" class="phx-message clearfix"> <div id="lvtttdx" class="content clearfix"> <div id="lvtttdx" class="item"> <span>標(biāo)題：</span> <div> <input name="txtMsgTitle" type="text" maxlength="100" id="txt_subject" class="txt"> 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