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data-src="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast" data-rel="http://y1.yzimgs.com/uploads/495139/2016620-143234402.png?watermark/2/text/5LiK5rW35bq35pyX55Sf54mp56eR5oqA5pyJ6ZmQ5YWs5Y-4/font/5a6L5L2T/fontsize/300/fill/I0E3QTlBOA==/gravity/SouthEast" data-title=""></a> </div> <a class="big_photo" href="/View_Image_2022155345.html" target="_blank">查看大圖</a> </div> <ul class="info_list"> <li><h1 class="info_name">產(chǎn)品名稱：人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒</h1></li> <li>產(chǎn)品型號(hào)：CA12</li> <li>產(chǎn)品廠商：KALANG</li> <li>產(chǎn)品文檔： </li> </ul> <ul class="tb-thumb thumblist"> <li id="wekauyyom" class="tb-selected"> <div id="wekauyyom" class="tb-pic tb-s40 img-wrapper" data-type="productsmall" 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人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒采用雙抗體夾心法。用抗人CA12抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)標(biāo)本或標(biāo)準(zhǔn)品中的CA12會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人CA12抗體和辣根過(guò)氧化物酶標(biāo)記的親和素。抗人CA12抗體與結(jié)合在包被抗體上的人CA12結(jié)合、生物素與親和素特異性結(jié)合而形成**復(fù)合物，游離的成分被洗去。加入發(fā)光底物混合液，發(fā)光底物在辣根過(guò)氧化物酶的催化下發(fā)出熒光，用化學(xué)發(fā)光**分析儀測(cè)定化學(xué)發(fā)光值（RLU），人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒濃度與化學(xué)發(fā)光值之間呈正相關(guān)，通過(guò)繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中CA12的濃度。 </div> </div> <div id="wekauyyom" class="details_info"> <div id="wekauyyom" class="title"> 詳情介紹： </div> <div id="wekauyyom" class="text yzeditor"> <p> <strong>人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒</strong> </p> <p> 基本原理： </p> <p> <span style="font-family:Verdana, Arial, Helvetica, sans-serif;line-height:20px;text-align:justify;text-indent:24px;white-space:normal;">本試劑盒<span style="font-family:Verdana, Arial, Helvetica, sans-serif;font-size:13.3333px;line-height:20px;text-align:justify;text-indent:26.6667px;white-space:normal;">采用雙抗體夾心法。用抗人CA12抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)標(biāo)本或標(biāo)準(zhǔn)品中的CA12會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人CA12抗體和辣根過(guò)氧化物酶標(biāo)記的親和素?？谷薈A12抗體與結(jié)合在包被抗體上的人CA12結(jié)合、生物素與親和素特異性結(jié)合而形成**復(fù)合物，游離的成分被洗去。加入發(fā)光底物混合液，發(fā)光底物在辣根過(guò)氧化物酶的催化下發(fā)出熒光，用化學(xué)發(fā)光**分析儀測(cè)定化學(xué)發(fā)光值（RLU），CA12濃度與化學(xué)發(fā)光值之間呈正相關(guān)，通過(guò)繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中CA12的濃度。</span></span> </p> <p> <span style="white-space:normal;"><strong style="white-space:normal;"><strong style="white-space:normal;"><strong>人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒</strong></strong></strong>描述：</span> </p> <p> <table class=" noBorderTable ke-zeroborder" align="center" border="0" cellspacing="1" width="640" style="margin:0px;padding:0px;color:#5D5D5D;font-family:Verdana, Arial, Helvetica, sans-serif;font-size:13.3333px;background-color:#A8C29F;"> <tbody style="margin:0px;padding:0px;"> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">英文名稱</span> </td> <td colspan="3" bgcolor="#ffffff" height="25" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">Human CA12 (Carbonic Anhydrase XII) CLIA Kit</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">中文名稱</span> </td> <td height="25" colspan="3" bgcolor="#ffffff" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">貨號(hào)</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">KL-H0533c</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">種屬</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">Human</span>/人 </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">規(guī)格</span> </td> <td height="25" colspan="3" bgcolor="#ffffff" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">96T/Kit (8*12 wells)</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">檢測(cè)方法</span> </td> <td height="25" colspan="3" bgcolor="#ffffff" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">雙抗體夾心法</span> </td> </tr> <tr style="margin:0px;padding:0px;"> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">檢測(cè)范圍</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">62.5~4000pg/mL</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">靈敏度</span> </td> <td bgcolor="#ffffff" height="25" width="160" style="margin:0px;padding:0px;"> <span id="wekauyyom" class="black" style="margin:0px;padding:0px;color:#000000;">37.5pg/mL</span> </td> </tr> </tbody> </table> </p> <p> <strong style="line-height:1.5;"><strong style="white-space:normal;">人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒</strong></strong><span style="line-height:1.5;">試劑盒組成：</span> </p> <p><table class=" <tbody> <tr> <td bgcolor="#ffffff" width="160"> <strong><span id="wekauyyom" </td> <td bgcolor="#ffffff" <strong><span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <strong><span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <strong><span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <br> </td> </tr> <tr> <td bgcolor="#ffffff" width="160"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="400"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="200"> <span id="wekauyyom" </td> <td bgcolor="#ffffff" width="160"> <br> </td> </tr> <tr> <td bgcolor="#ffffff"> <span id="wekauyyom" </td> <td bgcolor="#ffffff"> <span id="wekauyyom" </td> <td bgcolor="#ffffff"> <span id="wekauyyom" </td> <td bgcolor="#ffffff"> <br> </td> </tr> <tr> <td colspan="4" bgcolor="#ffffff"> <strong><span id="wekauyyom" <span id="wekauyyom" </td> </tr> </tbody> </table> <strong style="white-spac noBorderTable ke-zeroborder" align="center" border="0" cellspacing="1" width="650" style="margin:0px;padding:0px;color:#5D5D5D;font-family:Verdana, Arial, Helvetica, sans-serif;background-color:#A8C29F;"> class="black" style="color:#000000;">中文名稱</span></strong> height="25" width="400"> class="black" style="color:#000000;">英文名稱</span></strong> class="black" style="color:#000000;">規(guī)格</span></strong> class="black" style="color:#000000;">保存</span></strong> class="black" style="color:#000000;">CLIA酶標(biāo)板</span> class="black" style="color:#000000;">Micro CLIA Plate</span> class="black" style="color:#000000;">8×12 wells</span> class="black" style="color:#000000;">4℃/-20℃ #</span> class="black" style="color:#000000;">凍干標(biāo)準(zhǔn)品</span> class="black" style="color:#000000;">Reference Standard</span> class="black" style="color:#000000;">2 支</span> class="black" style="color:#000000;">4℃/-20℃ #</span> class="black" style="color:#000000;">標(biāo)準(zhǔn)品&樣品稀釋液</span> class="black" style="color:#000000;">Reference Standard & Sample Diluent</span> class="black" style="color:#000000;">1瓶 20mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">濃縮生物素化抗體</span> class="black" style="color:#000000;">Concentrated Biotinylated Detection Ab</span> class="black" style="color:#000000;">1支 120μL</span> class="black" style="color:#000000;">4℃/-20℃ #</span> class="black" style="color:#000000;">生物素化抗體稀釋液</span> class="black" style="color:#000000;">Biotinytated Detection Ab Diluent</span> class="black" style="color:#000000;">1瓶10mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">濃縮HRP酶結(jié)合物</span> class="black" style="color:#000000;">Concentrated HRP Conjugate</span> class="black" style="color:#000000;">1支 120μL</span> class="black" style="color:#000000;">4℃(避光)</span> class="black" style="color:#000000;">酶結(jié)合物稀釋液</span> class="black" style="color:#000000;">HRP Conjugate Diluent</span> class="black" style="color:#000000;">1瓶 10mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">濃縮洗滌液（25×）</span> class="black" style="color:#000000;">Concentrated Wash Buffer (25×)</span> class="black" style="color:#000000;">1瓶 30mL</span> class="black" style="color:#000000;">4℃</span> class="black" style="color:#000000;">發(fā)光底物A液</span> class="black" style="color:#000000;">Substrate Reagent A</span> class="black" style="color:#000000;">1瓶 5mL</span> class="black" style="color:#000000;">4℃(避光)</span> class="black" style="color:#000000;">發(fā)光底物B液</span> class="black" style="color:#000000;">Substrate Reagent B</span> class="black" style="color:#000000;">1瓶 5mL</span> class="black" style="color:#000000;">4℃(避光)</span> class="black" style="color:#000000;">封板覆膜</span> class="black" style="color:#000000;">Plate Sealer</span> class="black" style="color:#000000;">5 張</span> class="black" style="color:#000000;">產(chǎn)品說(shuō)明書</span> class="black" style="color:#000000;">Manual</span> class="black" style="color:#000000;">1 份</span> class="black" style="color:#000000;">質(zhì)檢報(bào)告</span> class="black" style="color:#000000;">Certificate of Analysis</span> class="black" style="color:#000000;">1 份</span> class="black" style="color:#000000;">特別說(shuō)明</span></strong>：<br> class="black" style="color:#000000;">#: 一周內(nèi)使用可存于4℃，需長(zhǎng)時(shí)間存放或多次使用建議存于-20℃.</span> e:normal;"><strong style="white-space:normal;">人碳酸酐酶XII(CA12)化學(xué)發(fā)光**分析試劑盒</strong></strong>操作步驟：</p> <p> 1.在各孔中加入標(biāo)準(zhǔn)品或樣品各 100μL，37℃孵育90分鐘<br> 2. 倒去孔內(nèi)液體，拍干，加入 100μL 生物素化抗體工作液，37℃孵育 60 分鐘<br> 3. 洗滌 3 次<br> 4. 加入 100μL 酶結(jié)合物工作液， 37℃孵育 30 分鐘<br> 5. 洗滌 5 次<br> 6. 加入 100μL 發(fā)光底物混合液， 37℃孵育 5 分鐘左右<br> 7. 立即測(cè)定各孔的化學(xué)發(fā)光值<br> 8. 結(jié)果計(jì)算 </p> <p> KL-FPN-Hu 人膜鐵轉(zhuǎn)運(yùn)蛋白(FPN) 檢測(cè)試劑盒 human Ferroportin (FPN) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.126ng/mL 96T 12 months<br> KL-DKK1-Ra 大鼠Dickkopf相關(guān)蛋白1(DKK1) 檢測(cè)試劑盒 Rat Dickkopf Related Protein 1 (DKK1) ELISA Kit Rattus norvegicus Rat 0.313-20ng/mL 0.133ng/mL 96T 12 months<br> KL-IL6R-Mu 小鼠白介素6受體(IL6R) 檢測(cè)試劑盒 mouse Interleukin 6 Receptor (IL6R) ELISA Kit Mus musculus mouse 31.25-2000pg/mL 11.4pg/mL 96T 12 months<br> KL-SFRP1-Ra 大鼠分泌型卷曲相關(guān)蛋白1(SFRP1) 檢測(cè)試劑盒 Rat Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.058ng/mL 96T 12 months<br> KL-EPX-Mu 小鼠嗜酸性粒細(xì)胞過(guò)氧化物酶(EPX) 檢測(cè)試劑盒 mouse Eosinophil Peroxidase (EPX) ELISA Kit Mus musculus mouse 31.25-2000pg/mL 12.9pg/mL 96T 12 months<br> KL-DCX-Ra 大鼠雙腎上腺皮質(zhì)**(DCX) 檢測(cè)試劑盒 Rat Doublecortin (DCX) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.054ng/mL 96T 12 months<br> KL-EPHB3-Ra 大鼠肝配蛋白B受體3(EPHB3) 檢測(cè)試劑盒 Rat Ephrin Type B Receptor 3 (EPHB3) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.067ng/mL 96T 12 months<br> KL-MTMR9-Ra 大鼠肌微管素相關(guān)蛋白9(MTMR9) 檢測(cè)試劑盒 Rat Myotubularin Related Protein 9 (MTMR9) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.063ng/mL 96T 12 months<br> KL-TRPM4-Ra 大鼠轉(zhuǎn)化受體電位陽(yáng)離子通道亞家族M成員4(TRPM4) 檢測(cè)試劑盒 Rat Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.055ng/mL 96T 12 months<br> KL-REPIN1-Hu 人復(fù)制啟動(dòng)因子1(REPIN1) 檢測(cè)試劑盒 human Replication Initiator 1 (REPIN1) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.129ng/mL 96T 12 months<br> KL-REPIN1-Mu 小鼠復(fù)制啟動(dòng)因子1(REPIN1) 檢測(cè)試劑盒 mouse Replication Initiator 1 (REPIN1) ELISA Kit Mus musculus mouse 0.313-20ng/mL 0.135ng/mL 96T 12 months<br> </p> </div> </div> </div> </div> </div> <div id="wekauyyom" class="appWrapper" id="app_33341434" role="draggable" data-type="app" data-appid="message" data-config="" 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