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id="qekaoycoa0" class="datum_info"> <h1 class="title">怎樣才能選到合適的酶標(biāo)板</h1> <div id="qekaoycoa0" class="bgcolor_01"> <div id="qekaoycoa0" class="bgcolor_sum"> <div id="qekaoycoa0" class="left">日期：2024-11-22 01:49</div> <div id="qekaoycoa0" class="right">瀏覽次數(shù)：1360</div> </div> <div id="qekaoycoa0" class="bgcolor_sum01"> 摘要：怎樣才能選到合適的酶標(biāo)板高結(jié)合力：外表經(jīng)處理后，其蛋白結(jié)合才能大大增強(qiáng)，可達(dá)300~400ngIgG/cm2，首要結(jié)合的蛋白分子量>10kD。運(yùn)用該類酶標(biāo)板可進(jìn)步敏感性，并可相對削減包被蛋白的濃度和用量，不足之處為較易發(fā)作非特異性反響?？乖蚩贵w包被后，以非離子去污劑無法有效地關(guān)閉未結(jié)合蛋白的部位，需運(yùn)用蛋白作為關(guān)閉劑。 </div> </div> <div id="qekaoycoa0" class="content"> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;"> <h1 style="margin:0px;padding:0px;font-size:20px;font-weight:400;color:#444444;font-family:"Microsoft YaHei";white-space:normal;background-color:#FBF9F2;"> 怎樣才能選到合適的酶標(biāo)板 </h1> </pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">一：依據(jù)酶標(biāo)板與蛋白和其它分子結(jié)合才能的不同，分為高結(jié)合力、中結(jié)合力和氨基化高結(jié)合力：外表經(jīng)處理后，其蛋白結(jié)合才能大大增強(qiáng)，可達(dá)300~400ngIgG/cm2，首要結(jié)合的蛋白分子量>10kD。</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">運(yùn)用該類酶標(biāo)板可進(jìn)步敏感性，并可相對削減包被蛋白的濃度和用量，不足之處為較易發(fā)作非特異性反響。抗原或抗</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">體包被后，以非離子去污劑無法有效地關(guān)閉未結(jié)合蛋白的部位，需運(yùn)用蛋白作為關(guān)閉劑。中結(jié)合力：經(jīng)外表疏水鍵被迫與蛋白結(jié)合，合適作為分子量>20kD的大分子蛋白的固相載體，其蛋白結(jié)合才能為</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">200~300ng IgG/cm2。該類板能夠慵懶蛋白或非離子去污劑作為關(guān)閉液。氨基化：經(jīng)外表改性處理后具有帶正電荷的氨基，其疏水鍵由親水鍵取代。該類酶標(biāo)板合適作為小分子蛋白的固相</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">載體。運(yùn)用合適的緩沖液和pH值，其外表可經(jīng)過離子鍵與帶負(fù)電荷的小分子結(jié)合。由于其外表的親水特性和可經(jīng)過</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">其它交聯(lián)劑共價(jià)結(jié)合的才能，可用于固定溶于Triton-100、Tween 20等去污劑的蛋白分子。該類板的缺點(diǎn)為由于</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">降低了疏水性，一部分蛋白分子無法結(jié)合;此外，其外表需有效地關(guān)閉。由于親水和共價(jià)的外表特性，運(yùn)用的關(guān)閉液</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">必須能夠與非反響性氨基基團(tuán)和所挑選的交聯(lián)劑中任何功用基團(tuán)發(fā)作效果。 </pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;"> </pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">二：依據(jù)酶標(biāo)板底部的不同，能夠分為平底、U型底、V型底平底的折射率低，適于在酶標(biāo)儀檢測；U型底的酶標(biāo)板折射率較高，便利加樣、吸樣、混勻等操作，能夠不用放在</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">酶標(biāo)儀上，直接經(jīng)過目測觀察色彩改變情況，然后斷定有無相應(yīng)的**反響。V底的酶標(biāo)板能夠準(zhǔn)確的汲取樣品。 </pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;"> </pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">三：依據(jù)色彩分為通明、黑色、白色通明的是*常用的，用于*一般的酶聯(lián)**試驗(yàn)。相對于通明的的板子，還有用于發(fā)光檢測用的不通明的板子，一</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">般有黑色和白色兩種。黑色的酶標(biāo)板本身會有光吸收，所以它的信號相對于白色的板子要低許多，因而一般用于檢</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">測較強(qiáng)的光，如熒光檢測。而白色的酶標(biāo)板就用于較弱的光檢測，常用于一般的化學(xué)發(fā)光。別的黑色的酶標(biāo)板還能</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">夠消弱非特異反響帶來的問題。有些客戶會問，用一般的酶標(biāo)板可不能夠進(jìn)行發(fā)光檢測，回答是不能夠的，由于一般</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">從化學(xué)發(fā)光反響中發(fā)射出的光是各向同性的，也就是說各個(gè)方向上平等發(fā)射出來的。如果用通明的板子，光不只會從</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">垂直方向發(fā)散，還從水平方向發(fā)散出來。很容易的就會經(jīng)過各個(gè)孔之間的空隙和孔壁。這樣的話，光較強(qiáng)時(shí)相鄰孔之</pre> <pre style="margin-top:0px;margin-bottom:0px;padding:0px;font-family:"Courier New", Courier, monospace;white-space:pre-wrap;font-size:14px;background-color:#FFFFFF;">間的影響就會很大，因而不能用通明的酶標(biāo)板來進(jìn)行化學(xué)發(fā)光試驗(yàn)。當(dāng)然，現(xiàn)在的酶標(biāo)板還有可拆開和不行拆開供客</pre> </div> <div id="qekaoycoa0" class="datumrelate"> <span>下一篇：</span> <a 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